Mutational and functional analysis of the [beta]-carotene ketolase involved in the production of canthaxanthin and astaxanthin

Article Abstract:

The class of natural pigments known as carotenoids is commercially used as food colorants and as antioxidants and the biosynthesis of the commercial carotenoids canthaxanthin and astaxanthin requires [beta]-carotene ketolase. Alanine-scanning mutagenesis was used to analyze the functional importance of the conserved amino acid residues of this enzyme from 'Paracoccus sp. strain N81106 and the result supported the hypothesis that the CrtW ketolase belongs to the family of iron-dependent integral membrane proteins.

United States, Carotenoids, Polymerase chain reaction, Mutagenesis, Chemical properties, Nucleotide sequencing

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Characterization of SafC, catechol 4-O-methyltransferase involved in saframycin biosynthesis

Article Abstract:

The study explains the synthesis and characterization of SafC, a catechol 4-O-methyltransferase protein, which is commonly used for the biosynthesis of various saframycin products. SafC has the ability to methylate a variety of catechols and hence can be used in many medical and biological applications.

Methyltransferases, Catechin

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